Constructive.Bio

nature

Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly 

Constructive Bio

Noncanonical Amino Acids Inspire the Development of Novel Drugs

Constructive Bio’s showcase HQ at The Recodery is formally opened 

Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid. Synonymous codon choice has diverse and important roles, and many synonymous substitutions are detrimental. Here we demonstrate that the number of codons used to encode the canonical amino acids can be reduced, through the genome-wide substitution of target codons by defined synonyms. We create a variant of Escherichia coli with a four-megabase synthetic genome through a high-fidelity convergent total synthesis. Our synthetic genome implements a defined recoding and refactoring scheme—with simple corrections at just seven positions—to replace every known occurrence of two sense codons and a stop codon in the genome. Thus, we recode 18,214 codons to create an organism with a 61-codon genome; this organism uses 59 codons to encode the 20 amino acids, and enables the deletion of a previously essential transfer RNA.

Total synthesis of Escherichia coli with a recoded genome

Constructive Bio have been selected for the Innovate UK Engineering Biology and Industrial Biotechnology Global Business Innovation Programme (GBIP) focused on San Jose, California USA.

Helena Francis PhD

Constructive Bio selected for the Innovate UK Engineering Biology and Industrial Biotechnology Global Business Innovation Programme (GBIP).

Biomachines Beyond Nature: How Can We Make Molecules Precisely Tailored to Our Needs?

Ola Wlodek PhD

Dr Ola Wlodek has been invited to present at SynBioBeta

Constructive Bio joins NVIDIA Inception, accelerating its work writing genomes from scratch and creating programmable biomolecules

Highlights[ 7 ]

Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly

Noncanonical Amino Acids Inspire the Development of Novel Drugs

Constructive Bio’s showcase HQ at The Recodery is formally opened

Total synthesis of Escherichia coli with a recoded genome

Constructive Bio selected for the Innovate UK Engineering Biology and Industrial Biotechnology Global Business Innovation Programme (GBIP).

Dr Ola Wlodek has been invited to present at SynBioBeta

Constructive Bio joins NVIDIA Inception, accelerating its work writing genomes from scratch and creating programmable biomolecules

Unlock the future! Join our newsletter for groundbreaking updates of Constructive.Bio

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Highlights[ 7 ]

Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly

Noncanonical Amino Acids Inspire the Development of Novel Drugs

Constructive Bio’s showcase HQ at The Recodery is formally opened

Total synthesis of Escherichia coli with a recoded genome

Constructive Bio selected for the Innovate UK Engineering Biology and Industrial Biotechnology Global Business Innovation Programme (GBIP).

Dr Ola Wlodek has been invited to present at SynBioBeta

Constructive Bio joins NVIDIA Inception, accelerating its work writing genomes from scratch and creating programmable biomolecules

Unlock the future! Join our newsletter for groundbreaking updates of Constructive.Bio

Sign up forOurNewsletter

Sign up for
OurNewsletter